Ultra-Rapid Biological Indicators for Steam Sterilizers, 20 Minute Results, Tuttnauer WTL198-0072 50/box

Tuttnauer Ultra-Rapid, Biological Indicators for Steam Sterilizers, 20 Minute Results, 50/box

Be compliant. Avoid long turn-around times on spore tests. Tuttnauer's new biological indicators prove sterilizer performance in just 20 minutes! Recommended for use with Tuttnauer MiniBio Auto-Reader.

Compatible with Class S Gravity and Class B Pre-Post Vacuum autoclaves.

Spore tests use endoscopes to test sterilization for all conditions to be met to kill a specified numbers of organisms.

Tuttnauer WTL198-0072

Features:
Self-contained biological indicator inoculated with viable 106 Geobacillus stearothermophilus bacterial spores
Spore Tests Results in 20 minutes!
On each BI is a chemical process indicator that changes color from pink to brown when exposed to steam
Easy to use and 100% accurate
50 biological indicators per pack



How to Use the BI-TUR biological indicator for steam sterilization:

1. Identify the WTL198-0072 SCBI by writing the sterilizer number (in case of having more than one), load number and processing date on the label.

2. Pack the SCBI along with materials to be sterilized in an appropriate package according to recommended sterilization practices. Place the package in those areas which are considered most inaccessible for the sterilizing agent (e.g., the center of the load and areas near the door).

3. Sterilize as usual.

4. After the sterilization process has finished, open the sterilizer door, wait five minutes and remove the SCBI from the package.
CAUTION: Wear safety glasses and gloves when removing the WTL198-0072 SCBI from the sterilized package. WARNING: Do not crush or handle the SCBI excessively, since this might cause the glass ampoule to burst. Let the SCBI cool down until it reaches room temperature.

5. Check the Process Indicator on SCBI label. A color change to brown indicates that the SCBI has been exposed to Steam.
IMPORTANT: this color change does not evidence the process effectiveness to achieve sterility. If the Process Indicator color has not changed, check the sterilization process.

6. Press the lid to seal the tube. Crush the ampoule contained in the SCBI with an individual ampoule crusher or with the ampoule crusher placed within the incubator´s incubation area. Then shake the tube down vigorously, with movements similar to those performed to lower the temperature in a mercury thermometer, until the medium reaches the base of the tube and soaks the spore carrier entirely. Finally, place the SCBI in the incubator. IMPORTANT: Use a non-sterilized SCBI as a positive control in order to ensure that correct incubation conditions were met; capability of medium to promote rapid growth; viability of spores has not been altered due to improper storage temperature, humidity or proximity to chemicals and proper functioning of Auto-Reader Incubators. Both, the positive control indicator and the processed indicator, should belong to the same batch.

7. Incubate the processed indicator and the positive control indicator in Auto-Reader Incubators for a maximum of 20 minutes at (60 ± 2) °C for ultra rapid readout.

Readout Times:
Ultra Rapid readout: 20 minutes
The Ultra Rapid Readout must be carried out in the Auto-Reader Incubators. Fluorescence is emitted after the reader stimulates the spore carrier with UV light. Final readout of negative results is readily available after 20 minutes of incubation. Fluorescence readout is an indirect measure of the germination and growth of Geobacillus stearothermophilus spores, which have survived the sterilization process (positive result). Furthermore, a failure in the sterilization process can also become evident by culture medium color change. Due to the high sensitivity of the fluorescence results at 20 minutes, conventional incubation for color change of WTL198-0072 is not an advantage.

Visual Confirmation: 48 hours

Optionally, you can perform a visual color-change confirmation after a 48-hour incubation. If the sterilization process has not been successful, culture medium will turn yellow during incubation at 60 ºC, indicating the presence of living spores. If sterilization was successful, culture medium will remain purple after incubation. The positive control must show a color change from purple to yellow for results to be valid.

Readout time
7 days
A 7-day readout is optional and may be routinely performed. This is an initial validation of the 20-minute readout. Fluorescence results are compared to the 7-day visual readouts.
NOTE: If 7-day readout is performed, a humidified environment will be required to prevent medium from drying out.